Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?

Aruna K Mora, Sufiyan Khan, Birija S Patro, Sukhendu Nath

Chemical communications (Cambridge, England) 2020 Nov 18

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DAPI is used extensively to identify and quantify DNA in cellular systems assuming its exclusive staining of nucleic acids. However, the present results show that DAPI has much higher affinity towards protein aggregates than DNA. Thus, the use of DAPI for the nucleic acid assay of cellular systems with protein aggregates may not be very reliable.

Citation

Aruna K Mora, Sufiyan Khan, Birija S Patro, Sukhendu Nath. Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates? Chemical communications (Cambridge, England). 2020 Nov 18;56(89):13844-13847