Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B.

Journal of neuroimmunology 2020 Dec 15

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To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Citation

Yukitoshi Takahashi, Shigeko Nishimura, Emiko Takao, Risa Kasai, Kaoru Enokida, Kuniko Ida, Masataka Fukuoka, Takayoshi Koike, Hiroo Omatsu, Tokito Yamaguchi, Shiho Takano, Tomoyuki Yoshida, Hisashi Mori. Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B. Journal of neuroimmunology. 2020 Dec 15;349:577427