Hydrogen-Deuterium Exchange Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins.

Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is, nowadays, an increasingly important technique in studying protein conformation and dynamics. This technique possesses the advantages of low sample consumption, less limitation in protein size, and relatively simple experimental workflow. An HDX-MS experiment typically includes the steps of sample preparation, HDX reaction, quenching of HDX reaction, protease digestion, and LC-MS analysis. Although HDX-MS has been an established technique and automatic sample handling devices are commercially available nowadays, proper experimental conditions of each step are crucial for a successful HDX-MS experiment. This chapter is to provide a general guideline for each step in the HDX-MS workflow and highlight some precautions needed to be taken in order to acquire useful conformational and dynamic information.

Citation

Pui-Kin So. Hydrogen-Deuterium Exchange Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins. Methods in molecular biology (Clifton, N.J.). 2021;2199:159-173

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PMID: 33125650

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