Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts.

Xiao Liu, Furong Xie, Guangyun Lai, Jun Wang

Archives of oral biology 2020 Dec

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We aimed to explore the role of Heterogeneous Nuclear Ribonucleoprotein L(hnRNP L) in enamel organ development through hnRNP L conditional knockout mice and knockdown of hnRNP L expression in mouse ameloblast-lineage cells (mALCs) METHODS: We created K14cre-mediated hnRNP L conditional knockout mice (hnRNP LK14/fl) and silenced the expression of hnRNP L in mALCs to investigate the role of hnRNP L in enamel organ development. We found that hnRNP LK14/fl mice presented enamel organ development defects with reduced number of inner enamel epithelium (IEE) cells. The proliferation and differentiation of the IEE cells/ameloblasts were suppressed. The cell proliferation and mineralization ability were also decreased after hnRNP L knockdown. Further studies showed that Bone Morphogenetic Protein (BMP) signaling pathway was attenuated after the knockdown of hnRNP L expression both in vivo and in vitro. These findings suggest that hnRNP L plays a critical role in enamel organ development by promoting the IEE cell/ameloblast proliferation and differentiation. BMP signaling pathway may be involved in the process. Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Citation

Xiao Liu, Furong Xie, Guangyun Lai, Jun Wang. Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts. Archives of oral biology. 2020 Dec;120:104933