Saori Yonebayashi, Kazuko Tajiri, Nobuyuki Murakoshi, Dongzhu Xu, Siqi Li, Duo Feng, Yuta Okabe, Zixun Yuan, Zonghu Song, Kazuhiro Aonuma, Akira Shibuya, Kazutaka Aonuma, Masaki Ieda
Journal of cellular and molecular medicine 2020 DecMacrophages are fundamental components of inflammation in post-myocardial infarction (MI) and contribute to adverse cardiac remodelling and heart failure. However, the regulatory mechanisms in macrophage activation have not been fully elucidated. Previous studies showed that myeloid-associated immunoglobulin-like receptor II (MAIR-II) is involved in inflammatory responses in macrophages. However, its role in MI is unknown. Thus, this study aimed to determine a novel role and mechanism of MAIR-II in MI. We first identified that MAIR-II-positive myeloid cells were abundant from post-MI days 3 to 5 in infarcted hearts of C57BL/6J (WT) mice induced by permanent left coronary artery ligation. Compared to WT, MAIR-II-deficient (Cd300c2-/- ) mice had longer survival, ameliorated cardiac remodelling, improved cardiac function and smaller infarct sizes. Moreover, we detected lower pro-inflammatory cytokine and fibrotic gene expressions in Cd300c2-/- -infarcted hearts. These mice also had less infiltrating pro-inflammatory macrophages following MI. To elucidate a novel molecular mechanism of MAIR-II, we considered macrophage activation by Toll-like receptor (TLR) 9-mediated inflammation. In vitro, we observed that Cd300c2-/- bone marrow-derived macrophages stimulated by a TLR9 agonist expressed less pro-inflammatory cytokines compared to WT. In conclusion, MAIR-II may enhance inflammation via TLR9-mediated macrophage activation in MI, leading to adverse cardiac remodelling and poor prognosis. © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
Saori Yonebayashi, Kazuko Tajiri, Nobuyuki Murakoshi, Dongzhu Xu, Siqi Li, Duo Feng, Yuta Okabe, Zixun Yuan, Zonghu Song, Kazuhiro Aonuma, Akira Shibuya, Kazutaka Aonuma, Masaki Ieda. MAIR-II deficiency ameliorates cardiac remodelling post-myocardial infarction by suppressing TLR9-mediated macrophage activation. Journal of cellular and molecular medicine. 2020 Dec;24(24):14481-14490
PMID: 33140535
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