Structural and biochemical studies of the glycosyltransferase Bs-YjiC from Bacillus subtilis.

Glycosylation possess prominent biological and pharmacological significance in natural product and drug candidate synthesis. The glycosyltransferase YjiC, discovered from Bacillus subtilis (Bs-YjiC), shows potential applications in drug development due to its wide substrate spectrums. In order to elucidate its catalytic mechanism, we solved the crystal structure of Bs-YjiC, demonstrating that Bs-YjiC adopts a typical GT-B fold consisting of a flexible N-domain and a relatively rigid C-domain. Structural analysis coupled with site-directed mutagenesis studies revealed that site Ser277 was critical for Nucleoside Diphosphate (NDP) recognition, while Glu317, Gln318, Ser128 and Ser129 were crucial for glycosyl moiety recognition. Our results illustrate the structural basis for acceptor promiscuity in Bs-YjiC and provide a starting point for further protein engineering of Bs-YjiC in industrial and pharmaceutical applications. Copyright © 2020 Elsevier B.V. All rights reserved.

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Bing Liu, Chang Zhao, Qianyin Xiang, Ninglin Zhao, Yunzi Luo, Rui Bao. Structural and biochemical studies of the glycosyltransferase Bs-YjiC from Bacillus subtilis. International journal of biological macromolecules. 2021 Jan 01;166:806-817

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PMID: 33152360

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