Wei Zhao, Ye Yuan, Burong Feng, Yue Sun, Huiwei Jiang, Wei Zhao, Yuyang Zheng, Lihui Zhao, Tingting Chen, Yan Bai, Pengzhou Hang, Yingfu Chen, Zhimin Du
Environmental toxicology and pharmacology 2021 JanZidovudine (3'-azido-2',3'-deoxythymidine; AZT) is a first-line drug for treatment of human immunodeficiency virus infection (HIV). However, its application is limited by cardiotoxicity due to cardiomyocyte injury. This study investigated whether Aloe-emodin (AE), an anthraquinone compound, protects against AZT-induced cardiomyocyte toxicity. MTT, JC-1 assays and TUNEL were examined to verify the protective effect of AE against AZT-induced cardiomyocyte injury. Western blotting was performed to explore the anti-apoptotic effect of AE using anti-apoptotic proteins p90rsk, p-bad, and bcl-2 and pro-apoptotic proteins apaf-1, cleaved-caspase-3, and cytochrome c. We observed a protective effect of AE against cell viability decrease and TUNEL positive cells increase induced by AZT, which was counteracted by BI-D1870. Western blot analysis found that AE significantly inhibited cardiomyocyte apoptosis by activating p90rsk/p-bad/bcl-2 signaling pathway. Furthermore, BI-D1870 counteracted the anti-apoptotic effect of AE. Taken together, these results indicate that AE attenuated AZT-induced cardiomyocyte apoptosis by activating p90rsk. Copyright © 2020. Published by Elsevier B.V.
Wei Zhao, Ye Yuan, Burong Feng, Yue Sun, Huiwei Jiang, Wei Zhao, Yuyang Zheng, Lihui Zhao, Tingting Chen, Yan Bai, Pengzhou Hang, Yingfu Chen, Zhimin Du. Aloe-emodin relieves zidovudine-induced injury in neonatal rat ventricular myocytes by regulating the p90rsk/p-bad/bcl-2 signaling pathway. Environmental toxicology and pharmacology. 2021 Jan;81:103540
PMID: 33161113
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