Extraction and Purification of DNA from Wood at Various Stages of Decay for Metabarcoding of Wood-Associated Fungi.

Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.

Citation

Jeff R Powell, Michaela Blyton, Brad Oberle, Gillian L Powell, Jessica Rigg, Darcy Young, Amy E Zanne. Extraction and Purification of DNA from Wood at Various Stages of Decay for Metabarcoding of Wood-Associated Fungi. Methods in molecular biology (Clifton, N.J.). 2021;2232:113-122

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PMID: 33161543

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