Ziyu Deng, Yaqiong Pei, Shishuai Wang, Bin Zhou, Xinyao Hou, Jing Li, Bin Li, Hongshan Liang
Journal of agricultural and food chemistry 2020 Nov 25There are multiple obstacles in the gastrointestinal tract (GIT) for oral administration of bioactive macromolecules. Here, we engineered an oral delivery vehicle (sporopollenin exine capsules with carboxymethylpachymaran (CMP)/metal ion modification) with targeted release based on food-grade ingredients and processing operations. Then, the interaction and binding mechanisms between CMP and metal ions in the vehicle were investigated. By using β-galactosidase (β-Gal) as a model protein, the systems were characterized for the surface morphology and monitored by the in vitro release profile of β-Gal. Notably, the CMP/metal ion systems not only markedly decreased the CMP dosage but also achieved a valid long-term release compared with the previously reported CMP system. Among all the systems, the CMP/3% AlCl3 system showed the best ability to control the release with the maximum residual activity of β-Gal at nearly 72% after 24 h of treatment. Subsequently, the interaction mechanism between CMP and metal ions within the system was characterized by the perspectives of microstructure, rheological properties, and spectroscopy characteristics. The results indicated that the low pH conditions are conducive to the further cross-linking of CMP and metal ions, resulting in a high gel strength and thus a dense structure, which can impact the controlled release of β-Gal in the GIT. Overall, the system may be utilized in the administration of medical and functional foods, specifically for the delivery of bioactive proteins via the oral route.
Ziyu Deng, Yaqiong Pei, Shishuai Wang, Bin Zhou, Xinyao Hou, Jing Li, Bin Li, Hongshan Liang. Designable Carboxymethylpachymaran/Metal Ion Architecture on Sunflower Sporopollenin Exine Capsules as Delivery Vehicles for Bioactive Macromolecules. Journal of agricultural and food chemistry. 2020 Nov 25;68(47):13990-14000
PMID: 33174430
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