Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1.

The Plant journal : for cell and molecular biology 2021 Feb

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IMPORTIN-α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin-α isoforms in Arabidopsis that recruit nuclear localization signal-containing cargo proteins to the nuclear import machinery. IMP-α3/MOS6 is required genetically for full autoimmunity of the nucleotide-binding leucine-rich repeat immune receptor mutant snc1 (suppressor of npr1-1, constitutive 1) and MOS6 also contributes to basal disease resistance. Here, we investigated the contribution of the other importin-α genes to both types of immune responses, and we analyzed potential interactions of all importin-α isoforms with SNC1. By using reverse-genetic analyses in Arabidopsis and protein-protein interaction assays in Nicotiana benthamiana, we provide evidence that among the nine α-importins in Arabidopsis, IMP-α3/MOS6 is the main nuclear transport receptor of SNC1, and that IMP-α3/MOS6 is required selectively for autoimmunity of snc1 and basal resistance to mildly virulent Pseudomonas syringae in Arabidopsis. © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

Citation

Daniel Lüdke, Charlotte Roth, Sieglinde A Kamrad, Jana Messerschmidt, Denise Hartken, Jonas Appel, Bojan F Hörnich, Qiqi Yan, Stefan Kusch, Melanie Klenke, Annette Gunkel, Lennart Wirthmueller, Marcel Wiermer. Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1. The Plant journal : for cell and molecular biology. 2021 Feb;105(4):994-1009