Methods to Study Integrin Functions on Exosomes.

Eiji Kawamoto, Eun Jeong Park, Motomu Shimaoka

Methods in molecular biology (Clifton, N.J.) 2021

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Exosomes represent an important group of extracellular vesicles. They are formed in endosomal compartments and are actively secreted to extracellular spaces. Several membrane proteins, including integrins, are present on the surface of exosomes. As exosomal integrins are competent for binding to ligand, they can play important roles in directing the tissue distribution of exosomes. Integrin-directed exosomal trafficking in vivo is involved in regulating the remodeling of cell homing niches for metastatic cancers and migrating lymphocytes. This chapter describes the methods used to study integrin functions on exosomes including: isolation and biophysical characterization of exosomes, exosomal integrin-ligand binding assays, and in vivo competitive exosome homing assays.

Citation

Eiji Kawamoto, Eun Jeong Park, Motomu Shimaoka. Methods to Study Integrin Functions on Exosomes. Methods in molecular biology (Clifton, N.J.). 2021;2217:265-281