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Endocytosis is a vesicle-based mechanism by which eukaryotic cells internalize extracellular material. There are several types of this universal mechanism linked to different types of endocytosed cargo, including pathogens; therefore, several approaches can be applied. Here, we describe techniques that are applicable to study the internalization of flaviviruses; dextrans; transporters, such as, glutamate transporter vGlut1; and peptidergic signaling molecules, including atrial natriuretic peptide into astrocytes, the most heterogeneous neuroglial cells, which play a key homeostatic role in the central nervous system.


Maja Potokar, Jernej Jorgačevski, Robert Zorec. Methods for Monitoring Endocytosis in Astrocytes. Methods in molecular biology (Clifton, N.J.). 2021;2233:93-100

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PMID: 33222129

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