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As a macrolide antibiotic, clarithromycin (CLA) has a high detection rate in surface water and sewage treatment plant effluents worldwide, posing a considerably high ecological risk to aquatic ecosystem. However, algal transcriptome and metabolome in response to CLA remains largely unknown. In this study, a model alga Raphidocelis subcapitata (R. subcapitata), was exposed to CLA at the concentrations of 0, 3, 10, and 15 μg L-1. Transcriptomic analysis was performed for all the treatment groups, whereas metabolomics was merely applied to 0, 3, and 10 μg L-1 groups because of the limited amount of algal biomass. After 7 d cultivation, the growth of R. subcapitata was significantly hindered at the concentrations above 10 μg L-1. A total of 115, 1833, 2911 genes were differentially expressed in 3, 10, and 15 μg L-1 groups, respectively; meanwhile, 134 and 84 differentially accumulated metabolites (DAMs) were found in the 3 and 10 μg L-1 groups. Specifically, expression levels of DEGs and DAMs related to xenobiotic metabolism, electron transport and energy synthesis were dysregulated, leading to the produced reactive oxygen species (ROS). To confront the CLA-induced injury, the biosynthesis of unsaturated fatty acids and carotenoids of R. subcapitata in 3 μg L-1 were up-regulated; although the photosynthesis was up-regulated in both 10 μg L-1 and 15 μg L-1 groups, the energy synthesis and the ability to resist ROS in these two groups were down-regulated. Overall, this study shed light on the mechanism underlying the inhibitory effects of macrolide antibiotics in algae. Copyright © 2020 Elsevier Ltd. All rights reserved.


Jianglin Peng, Jiahua Guo, Yuan Lei, Jiezhang Mo, Haotian Sun, Jinxi Song. Integrative analyses of transcriptomics and metabolomics in Raphidocelis subcapitata treated with clarithromycin. Chemosphere. 2021 Mar;266:128933

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PMID: 33223212

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