Correlation Engine 2.0
Clear Search sequence regions

  • ANO1 (2)
  • catheter (1)
  • cation (2)
  • cellular structures (1)
  • CGRP (2)
  • hc030031 (4)
  • nahs (2)
  • peristalsis (1)
  • protamines (2)
  • purines (2)
  • random (1)
  • rat (2)
  • signals (1)
  • SMA1 (1)
  • suggests (1)
  • TRPA1 (13)
  • trpa1 protein, rat (1)
  • ureter (8)
  • ureter function (1)
  • ureter peristalsis (5)
  • urothelium (1)
  • α actin (1)
  • Sizes of these terms reflect their relevance to your search.

    The current study aimed to explore the expression of transient receptor potential A1 ion channels (TRPA1) in the rat ureter and to assess if TRPA1-active compounds modulate ureter function. The expression of TRPA1 in rat ureter tissue was studied by immunofluorescence. The TRPA1 distribution was compared to calcitonin gene-related peptide (CGRP), α-actin (SMA1), anoctamin-1 (ANO1), and c-kit. For in vivo analyses, a catheter was implanted in the right ureter of 50 rats. Ureter peristalsis and pressures were continuously recorded by a data acquisition set-up during intraluminal infusion of saline (baseline), saline plus protamine sulfate (PS; to disrupt the urothelium), saline plus PS with hydrogen sulfide (NaHS) or cinnamaldehyde (CA). Comparisons were made between rats treated systemically with vehicle or a TRPA1-antagonist (HC030031). TRPA1-immunoreactive nerves co-expressed CGRP and were mainly located in the suburothelial region of the ureter. Immunoreactivity for TRPA1 was also encountered in c-kit-positive but ANO1-negative cells of the ureter suburothelium and wall. In vivo, HC030031-treated rats had elevated baseline peristaltic frequency (p < 0.05) and higher intraluminal pressures (p < 0.01). PS increased the frequency of ureter peristalsis versus baseline in vehicle-treated rats (p < 0.001) but not in HC030031-treated rats. CA (p < 0.001) and NaHS (p < 0.001) decreased ureter peristalsis. This was counteracted by HC030031 (p < 0.05 and p < 0.01). In rats, TRPA1 is expressed on cellular structures considered of importance for peristaltic and mechanoafferent functions of the ureter. Functional data indicate that TRPA1-mediated signals regulate ureter peristalsis. This effect was pronounced after mucosal disruption and suggests a role for TRPA1 in ureter pathologies involving urothelial damage. © 2020 The Authors. Neurourology and Urodynamics published by Wiley Periodicals LLC.


    Philipp Weinhold, Luca Villa, Frank Strittmatter, Christian Gratzke, Christian G Stief, Fabio Castiglione, Francesco Montorsi, Petter Hedlund. The transient receptor potential A1 ion channel (TRPA1) modifies in vivo autonomous ureter peristalsis in rats. Neurourology and urodynamics. 2021 Jan;40(1):147-157

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 33232544

    View Full Text