Correlation Engine 2.0
Clear Search sequence regions

  • amyloid (3)
  • apoptosis (9)
  • aβ25 35 (1)
  • behavior (1)
  • brain (1)
  • gene (1)
  • Homeodomain Proteins (2)
  • humans (3)
  • LDH (1)
  • malondialdehyde (1)
  • mice (2)
  • micrornas (3)
  • mirn375 microrna, human (1)
  • neurons (3)
  • oxygen (1)
  • peptides (2)
  • protein human (2)
  • rna (3)
  • signal (1)
  • SIX4 (8)
  • Six4 protein (2)
  • species (1)
  • WT1 (18)
  • wt1 proteins (4)
  • Sizes of these terms reflect their relevance to your search.

    To study the effect of lncRNA WT1-AS on oxidative stress injury (OSI) and apoptosis of neurons in Alzheimer's disease (AD) and its specific mechanisms related to the microRNA-375 (miR-375)/SIX4 axis and WT1 expression. After bioinformatic prediction, WT1-AS was found to be downregulated in Aβ25-35treated SH-SY5Y cells, and WT1-AS overexpression inhibited WT1 expression. WT1 could target miR-375 to promote its expression. miR-375 bound to SIX4, and miR-375 overexpression inhibited SIX4 expression. WT1-AS inhibited OSI and apoptosis, while WT1 and miR-375 overexpression or SIX4 silencing reversed the WT1-AS effect on OSI and apoptosis. In vivo experiments revealed that WT1-AS improved learning/memory abilities and inhibited OSI and apoptosis in AD mice. Overexpression of WT1-AS can inhibit the miR-375/SIX4 axis, OSI and neuronal apoptosis in AD by inhibiting WT1 expression. Related lncRNAs were identified, and miR-375 downstream targets were predicted. WT1-AS, WT1, miR-375 and SIX4 expression was detected in a cell model induced by Aβ25-35. The binding of WT1 with miR-375 and that of miR-375 with SIX4 were further confirmed. Adenosine triphosphate (ATP), reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) activities, and apoptosis levels were tested after mitochondrial membrane potential observation. Learning/memory abilities and neuronal apoptosis were tested in a mouse model.


    Quanbao Wang, Xiumin Ge, Jie Zhang, Licheng Chen. Effect of lncRNA WT1-AS regulating WT1 on oxidative stress injury and apoptosis of neurons in Alzheimer's disease via inhibition of the miR-375/SIX4 axis. Aging. 2020 Nov 21;12(23):23974-23995

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 33234729

    View Full Text