Synthesis and immunogenicity of Brucella monovalent neoglycoconjugate.

Tiantian Hao, Haomeng Wang, Jiacheng Liu, Qian Zhang, Yuning Liu, Lianbo Zhao, Tao Zhu, Peng Yu, Na Guo, Xin Meng

Carbohydrate research 2021 Jan

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Brucellosis is a highly infectious zoonotic disease caused by Brucella. It is necessary to control and eliminate brucellosis. The cell wall O-polysaccharides of pathogenic Brucella species are homopolymers of the rare sugar 4,6-dideoxy-4-formamido-α-d-mannopyranose. Herein, one neoglycoconjugate was successfully synthesized based on disaccharide [Rha4NFo(1 → 2)Rha4NFo] as epitope. Disaccharide specific antibodies were detected by ELISA and the immune protective effect was further evaluated with PBS as control. The result showed that the synthetic neoglycoconjugate can produce moderate immune responses in mice and significantly decreased splenic Brucella M5 burden comparing with control group. The chemically defined antigen identified the A antigenic determinant and provided a structural basis for understanding the fine specificity of polyclonal antibodies that bind the A antigen. The neoglycoconjugate shows the potential in detection reagent or vaccine development for brucellosis. Copyright © 2020. Published by Elsevier Ltd.

Citation

Tiantian Hao, Haomeng Wang, Jiacheng Liu, Qian Zhang, Yuning Liu, Lianbo Zhao, Tao Zhu, Peng Yu, Na Guo, Xin Meng. Synthesis and immunogenicity of Brucella monovalent neoglycoconjugate. Carbohydrate research. 2021 Jan;499:108196