Regiospecific Synthesis of Calcium-Independent Daptomycin Antibiotics using a Chemoenzymatic Method.

Nagaraju Mupparapu, Yu-Hsin Cindy Lin, Tae Ho Kim, Sherif I Elshahawi

Chemistry (Weinheim an der Bergstrasse, Germany) 2021 Feb 24

filter terms:

Daptomycin (DAP) is a calcium (Ca2+ )-dependent FDA-approved antibiotic drug for the treatment of Gram-positive infections. It possesses a complex pharmacophore hampering derivatization and/or synthesis of analogues. To mimic the Ca2+ -binding effect, we used a chemoenzymatic approach to modify the tryptophan (Trp) residue of DAP and synthesize kinetically characterized and structurally elucidated regiospecific Trp-modified DAP analogues. We demonstrated that the modified DAPs are several times more active than the parent molecule against antibiotic-susceptible and antibiotic-resistant Gram-positive bacteria. Strikingly, and in contrast to the parent molecule, the DAP derivatives do not rely on calcium or any additional elements for activity. © 2020 Wiley-VCH GmbH.

Citation

Nagaraju Mupparapu, Yu-Hsin Cindy Lin, Tae Ho Kim, Sherif I Elshahawi. Regiospecific Synthesis of Calcium-Independent Daptomycin Antibiotics using a Chemoenzymatic Method. Chemistry (Weinheim an der Bergstrasse, Germany). 2021 Feb 24;27(12):4176-4182