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Accurate discrimination of common glycosaminoglycans (GAGs) before they are used in clinics is of great importance. Herein, a ratiometric sensor array Py-PP for discrimination of GAGs was constructed using three pyrene-porphyrin supramolecular complexes termed Py-PP1, Py-PP2 and Py-PP4. These complexes were readily synthesized by mixing pyrene-1-butyric acid (Py) and porphyrins PP1, PP2 and PP4 respectively. In the presence GAGs, the effective FRET from Py to porphyrin in the complex was influenced as a result of the competitive binding interactions between porphyrin and GAG. Controlled by the structural differences in the three porphyrins, complexes Py-PP1, Py-PP2 and Py-PP4 were determined to be cross-responsive towards tested GAGs including Hep, HA, Chs and DS. Distinctive fluorescence patterns were successfully generated for each GAG by the sensor array. The Py-PP sensor array was found to be powerful for discrimination of GAGs in both PBS and 5% serum media. Moreover, Py-PP was also successfully applied for reliable differentiation of Hep from other biological interferences and detection of trace GAG contaminants (0.1%, wt%) in Hep with 100% accuracy. Copyright © 2020 Elsevier B.V. All rights reserved.


Meiting Hou, Liangfei Fan, Xia Fan, Xin Liang, Weihua Zhang, Yubin Ding. Pyrene-porphyrin based ratiometric fluorescent sensor array for discrimination of glycosaminoglycans. Analytica chimica acta. 2021 Jan 02;1141:214-220

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PMID: 33248655

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