Molecular characterization and functional analysis of cyp11a and cyp11b in black rockfish (Sebastes schlegelii).

Minmin Sun, Shipeng Jiang, Weihao Song, Jiangbo Qu, Jie Qi

Journal of fish biology 2021 Jul

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The cyp11 includes cyp11a and cyp11b in most mammals and teleosts, encoded cholesterol side chain lyase and 11β-hydroxylase, respectively. It is essential in steroid hormone synthesis. However, studies on the regulation of cyp11 are limited, especially in teleosts. In this study, the molecular characterization and function of cyp11a and cyp11b of black rockfish was investigated. Both of them showed high homology with other teleost counterparts by phylogenetic analysis. The expression of cyp11a and cyp11b exhibited a clear sexually dimorphic pattern, with a higher expression level in testis than that of in ovaries. During the different developmental stages (40 dpf, 80 dpf, 190 dpf, 360 dpf, 720 dpf), the expression of cyp11a was earlier than cyp11b. In situ hybridization results showed that cyp11a and cyp11b were mainly expressed in oogonia and oocytes of the ovary. They were located in spermatogonia and interstitial compartment in the 1.5-year-old gonads, and spermatocytesgonia and the peritubular myoid cell of the testis in the 2.5-year-old gonads. To explore the distinct roles of cyp11a and cyp11b in gonads, oestrogen and androgens were used to stimulate the primary testicular and ovarian cells. The expressions of cyp11a and cyp11b were tested under different dose of 17α-methyltestosterone (17α-MT) and 17β-estradiol (E2). The results showed cyp11a was significantly increased at 10-6 mol ml-1 of 17α-MT and 10-8 mol ml-1 of E2 in ovary and 10-10 mol ml-1 of 17α-MT and E2 in testis, while cyp11b was significantly decreased after 17α-MT and E2 treatment. These results indicated that cyp11a and cyp11b were likely to have different functions, and also implied they might play an important roles in the differentiation of gonads and the synthesis of steroids in black rockfish. © 2020 Fisheries Society of the British Isles.

Citation

Minmin Sun, Shipeng Jiang, Weihao Song, Jiangbo Qu, Jie Qi. Molecular characterization and functional analysis of cyp11a and cyp11b in black rockfish (Sebastes schlegelii). Journal of fish biology. 2021 Jul;99(1):9-17