MicroRNA-107 inhibits the proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1].

Chaoping Huang, Yi Wang, Shi Huang, Zhenxiao Wang, Liangfa Liu

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery 2020 Oct

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Objective:To detect the expression of microRNA-107 （miR-107） and the calcium channel protein gene CACNA2D1 in laryngeal cancer tissues, to investigate the targeting relationship between miR-107 and CACNA2D1, and to analyze the effects of miR-107 on the proliferation, invasion and colony forming ability of laryngeal cancer cells. Method:Laryngeal cancer tissues and normal adjacent tissue samples from 40 patients with laryngeal cancer were collected, and qRT-PCR was used to detect the expression of miR-107 and CACNA2D1; Western Blot assay to detect the expression of α2δ1 in the above two tissues; the dual-luciferase reporter gene was used to detect the regulatory effect of miR-107 on CACNA2D1; after overexpression or knockdown of miR-107 in human laryngeal cancer cells TU212 and TU686, changes in the proliferation, clone formation, and invasion ability of laryngeal cancer cells were detected. Result:The expression of miR-107 in laryngeal cancer tissues was significantly lower than that in adjacent normal tissues, while the expression of CACNA2D1 was just the opposite, the difference was statistically significant （P<0.05）; the expression of α2δ1 in laryngeal cancer tissues is significantly higher than in normal tissues（P<0.05）; dual-luciferase reporter experiments confirmed that miR-107 binds to the 3'-UTR （202-209, 902-908） of the CACNA2D1 mRNA, thereby inhibiting the expression of CACNA2D1 and its biological effects; cell experiments showed that the proliferation, invasion, and clone formation of laryngeal cancer cells were significantly reduced after miR-107 overexpression （P<0.05）, and the cell proliferation, clone formation, and invasion were significantly enhanced after miR-107 was knocked down （P<0.05） . Conclusion:miR-107 inhibits the proliferation, clone formation, and invasion of laryngeal cancer cells by targeting CACNA2D1. Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.

Citation

Chaoping Huang, Yi Wang, Shi Huang, Zhenxiao Wang, Liangfa Liu. MicroRNA-107 inhibits the proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1]. Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery. 2020 Oct;34(10):911-918