Macrophage polarization induced by sustained release of 7,8-DHF from aligned PLLA fibers potentially for neural stem cell neurogenesis.

Qiao Xiao, Ting Guo, Jun Li, Liming Li, Kaixin Chen, Libing Zhou, Wutian Wu, Kwok-Fai So, Seeram Ramakrishna, Bin Liu, Limin Rong, Guoqiang Chen, Xiwen Xing, Liumin He

Materials science & engineering. C, Materials for biological applications 2021 Jan

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Neural stem cells (NSCs)-based regenerative medicine provides unprecedented therapeutic potential in neural insults. However, NSC-based neurogenesis is strongly influenced by the inflammatory environment after injury, which is mainly modulated by macrophages' secretion effects. In this study, we adopted poly L-lactic acid (PLLA) aligned fibers to guide macrophages elongating along the fiber directions and polarizing phenotypically toward anti-inflammatory M2 type. 7,8-DHF was loaded within the fibers with a sustained and controlled release pattern to promote the polarization of the macrophages and secretion of various anti-inflammatory factors. NSCs showed enhanced neuronal differentiation in the presence of the conditioned medium (CM) from M2 macrophages cultured on the 7,8-DHF-loaded PLLA aligned fibers. Moreover, M2-CM promoted neurogenesis by enhancing neurite outgrowth of NSC-derived neurons. In summary, we provided a novel therapeutic strategy for NSC neurogenesis by manipulating macrophage classification into anti-inflammatory M2 phenotypes with the 7,8-DHF-loaded PLLA aligned fibers, existing potential applications in treating neural injuries. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Qiao Xiao, Ting Guo, Jun Li, Liming Li, Kaixin Chen, Libing Zhou, Wutian Wu, Kwok-Fai So, Seeram Ramakrishna, Bin Liu, Limin Rong, Guoqiang Chen, Xiwen Xing, Liumin He. Macrophage polarization induced by sustained release of 7,8-DHF from aligned PLLA fibers potentially for neural stem cell neurogenesis. Materials science & engineering. C, Materials for biological applications. 2021 Jan;118:111415