Farhad Sabbaghi, Lorenz Ullner, Toszka Bohn, Jennifer Hahlbrock, Tobias Bopp, Edgar Schmitt, Matthias Klein, Michael Stassen
Journal of immunology (Baltimore, Md. : 1950) 2021 Jan 01IL-9 has lent its numerical designation to the Th9 subset of CD4+ Th cells, although it is also produced by additional cell types, including mast cells. It is a pleiotropic cytokine involved in allergic reactions, parasitic infections, autoimmune inflammation, and cancer immunity. In this article, we provide evidence that NFATc2 has contradictory functions in the expression of IL-9 in murine Th9 cells and bone marrow-derived mast cells (BMMC). The basis for this is our observation that the production of IL-9 in NFATc2-deficient Th9 cells is increased, whereas it is decreased in BMMC devoid of NFATc2. In addition, NFATc2 deficiency almost completely abrogates the expression of IL-3 in both cell types. However, selectively in BMMC, the production of IL-9 critically depends on autocrine IL-3 acting via the sustained activation of STAT5 on the expression of IL-9. Furthermore, we demonstrate that IL-3 acts independently and synergistically with IL-1β on the production of IL-9. Taken together, we highlight NFATc2-driven production of autocrine IL-3 as a critical and cell type-specific component for IL-9 expression in BMMC. Copyright © 2020 by The American Association of Immunologists, Inc.
Farhad Sabbaghi, Lorenz Ullner, Toszka Bohn, Jennifer Hahlbrock, Tobias Bopp, Edgar Schmitt, Matthias Klein, Michael Stassen. In Activated Murine Mast Cells, NFATc2 Is Critical for the Production of Autocrine IL-3, Thereby Promoting the Expression of IL-9. Journal of immunology (Baltimore, Md. : 1950). 2021 Jan 01;206(1):67-76
PMID: 33268486
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