Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein.

Xiaohui Li, Chunhua Zhang

Methods in molecular biology (Clifton, N.J.) 2021

filter terms:

Differential scanning fluorimetry (DSF) can be used to detect the binding of a small molecule ligand to a purified target protein. Upon binding with certain ligands, the protein can be stabilized from thermal denaturation. DSF uses a fluorescent dye and Real-Time PCR instrument to detect the unfolding process of proteins during thermal denaturation. The experiment can be set up and finished in 1 day once the purified protein is available.

Citation

Xiaohui Li, Chunhua Zhang. Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein. Methods in molecular biology (Clifton, N.J.). 2021;2213:183-186