Gaurav Sharma, Andrew I Yao, Gregory T Smaldone, Jennifer Liang, Matt Long, Marc T Facciotti, Mitchell Singer
Genomics 2021 JanTo accurately identify the genes and pathways involved in the initiation of the Myxococcus xanthus multicellular developmental program, we have previously reported a method of growing vegetative populations as biofilms within a controllable environment. Using a modified approach to remove up to ~90% rRNAs, we report a comprehensive transcriptional analysis of the M. xanthus developmental cycle while comparing it with the vegetative biofilms grown in rich and poor nutrients. This study identified 1522 differentially regulated genes distributed within eight clusters during development. It also provided a comprehensive overview of genes expressed during a nutrient-stress response, specific development time points, and during development initiation and regulation. We identified several differentially expressed genes involved in key central metabolic pathways suggesting their role in regulating myxobacterial development. Overall, this study will prove an important resource for myxobacterial researchers to delineate the regulatory and functional pathways responsible for development from those of the general nutrient stress response. Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Gaurav Sharma, Andrew I Yao, Gregory T Smaldone, Jennifer Liang, Matt Long, Marc T Facciotti, Mitchell Singer. Global gene expression analysis of the Myxococcus xanthus developmental time course. Genomics. 2021 Jan;113(1 Pt 1):120-134
PMID: 33276008
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