Portable and sensitive Ag nanorods based SERS platform for rapid HIV-1 detection and tropism determination.

Sarjana Yadav, Sneha Senapati, Dipen Desai, Shashank Gahlaut, Smita Kulkarni, J P Singh

Colloids and surfaces. B, Biointerfaces 2021 Feb

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In this study, surface-enhanced Raman scattering (SERS) based field-deployable platform has been explored for early detection and distinction of the human immunodeficiency virus (HIV-1). A highly optimized silver nanorods array, fabricated using glancing angle deposition technique was used as SERS substrate. Distinct signature peaks for varying concentrations (102 to 106 copies/mL) were identified in five different HIV-1 subtypes (A, B, C, D, and CRF02_AG). Binding of viruses directly with Ag nanorods without using antibodies or intermediate reagents is shown. The purified viruses were spiked in water and healthy plasma to capture pure HIV-1 peaks. Distinct peaks were also captured for the X4 and R5 tropic strains suggesting tropism based detection. The above data was further confirmed and analyzed statistically using a multivariate tool. Thus, the present study indicates the ability of the SERS platform to detect and differentiate the HIV-1 virus implying its further validation using clinical specimens and isolates. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Sarjana Yadav, Sneha Senapati, Dipen Desai, Shashank Gahlaut, Smita Kulkarni, J P Singh. Portable and sensitive Ag nanorods based SERS platform for rapid HIV-1 detection and tropism determination. Colloids and surfaces. B, Biointerfaces. 2021 Feb;198:111477