Is infrared spectroscopy combined with multivariate analysis a promising tool for heparin authentication?

Natalia A Burmistrova, Polina M Soboleva, Yulia B Monakhova

Journal of pharmaceutical and biomedical analysis 2021 Feb 05

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The investigation of the possibility to determine various characteristics of powder heparin (n = 115) was carried out with infrared spectroscopy. The evaluation of heparin samples included several parameters such as purity grade, distributing company, animal source as well as heparin species (i.e. Na-heparin, Ca-heparin, and heparinoids). Multivariate analysis using principal component analysis (PCA), soft independent modelling of class analogy (SIMCA), and partial least squares - discriminant analysis (PLS-DA) were applied for the modelling of spectral data. Different pre-processing methods were applied to IR spectral data; multiplicative scatter correction (MSC) was chosen as the most relevant. Obtained results were confirmed by nuclear magnetic resonance (NMR) spectroscopy. Good predictive ability of this approach demonstrates the potential of IR spectroscopy and chemometrics for screening of heparin quality. This approach, however, is designed as a screening tool and is not considered as a replacement for either of the methods required by USP and FDA. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Natalia A Burmistrova, Polina M Soboleva, Yulia B Monakhova. Is infrared spectroscopy combined with multivariate analysis a promising tool for heparin authentication? Journal of pharmaceutical and biomedical analysis. 2021 Feb 05;194:113811