Correlation Engine 2.0
Clear Search sequence regions


  • 4 and (1)
  • 7 aminoactinomycin d (13)
  • adult (1)
  • allophycocyanin (1)
  • amino acid (1)
  • analysis data (1)
  • annexin v (1)
  • anti- antibodies (1)
  • antibodies (3)
  • b cell lymphoma (1)
  • calcein am (9)
  • calcium (1)
  • cat (2)
  • cell death (8)
  • cells islets (26)
  • cellular (8)
  • cold (2)
  • control group (1)
  • culture (57)
  • culture media (23)
  • cyclophilin (1)
  • danger (3)
  • dead (4)
  • delta cells (8)
  • disease risk (1)
  • dnase (1)
  • donors (6)
  • dye (10)
  • edta (1)
  • euthanasia (2)
  • f 12 (1)
  • factor (1)
  • FADD (1)
  • fibrosarcoma (1)
  • fig alpha (1)
  • fitc (1)
  • gentamicin (1)
  • glucagon (5)
  • GLUT2 (22)
  • growth factors (3)
  • h 31 (1)
  • h 33 (1)
  • h 35 (1)
  • h 40 (1)
  • h 46 (1)
  • h 48 (1)
  • h 89 (1)
  • hair (2)
  • health status (1)
  • heparin (1)
  • hepes (1)
  • hiv 1 (1)
  • homeostasis (1)
  • human (8)
  • hyperglycemia (1)
  • hypoglycemia (2)
  • ibmx (3)
  • ice (4)
  • improves (1)
  • insulin (55)
  • insulinoma (1)
  • islet transplants (1)
  • islets (124)
  • ketoacidosis (1)
  • lab (1)
  • mass (2)
  • mice (1)
  • molecular probes (1)
  • necroptosis (7)
  • necrostatin- 1 (142)
  • nitric oxide (3)
  • north america (1)
  • novus (2)
  • oxygen (1)
  • pancreas (1)
  • past (1)
  • patients (5)
  • period (3)
  • phase (1)
  • phycoerythrin (2)
  • picogreen (1)
  • progenitor cells (1)
  • propidium (2)
  • rat (1)
  • research (2)
  • RIP1 (4)
  • serum (2)
  • serum albumin (1)
  • Somatostatin (5)
  • sonics (2)
  • stainless steel (1)
  • t lymphocytes (1)
  • toxic actions (1)
  • treatment pain (1)
  • triton x- 100 (1)
  • trolox (1)
  • under anesthesia (1)
  • uric acid (1)
  • Sizes of these terms reflect their relevance to your search.

    Previous studies have shown that necrostatin-1 (Nec-1) supplementation improved the viability of murine islets following exposure to nitric oxide, increased the survival of human islets during hypoxic culture, and augmented the maturation of pre-weaned porcine islets (PPIs) after 7 days of tissue culture. A limitation of these studies is that only one concentration of Nec-1 was used, and no studies have determined the optimal dose of Nec-1 for PPIs. Thus, the present study examined the effects of Nec-1 on PPIs at four different doses-0, 25, 50, 100, and 200 μM-after 7 days of tissue culture when supplemented on day 3. PPIs were isolated from pancreata of pre-weaned Yorkshire piglets (8-15 days old) and cultured in a specific islet maturation media added with Nec-1 on day 3 of tissue culture at 4 different doses-0, 25, 50, 100, and 200 μM (n = 6 for each dose). After 7 days of tissue culture, islets were assessed for recovery, viability, endocrine cellular content, GLUT2 expression in beta cells, and insulin secretion after glucose challenge. Nec-1 did not affect the viability of both intact islets and dissociated islets cells during tissue culture regardless of doses. Islets cultured in media supplemented with Nec-1 at 100 μM, but not 25, 50, or 200 μM, had a significantly higher recovery, composition of endocrine cells, GLUT2 expression in beta cells, and insulin secretion capacity than control islets cultured in media without Nec-1 supplementation. Moreover, culturing islets in 200 μM Nec-1 supplemented media not only failed to improve the insulin release but resulted in a lower glucose-induced insulin stimulation index compared to islets cultured in media added with 100 μM Nec-1. Xenotransplantation using porcine islets continues to demonstrate scientific advances to justify this area of research. Our findings indicate that Nec-1 supplementation at 100 μM was most effective to enhance the in vitro maturation of PPIs during tissue culture.

    Citation

    Hien Lau, Nicole Corrales, Samuel Rodriguez, Colleen Luong, Mohammadreza Mohammadi, Veria Khosrawipour, Shiri Li, Michael Alexander, Paul de Vos, Jonathan R T Lakey. Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets. PloS one. 2020 Dec 07;15(12):e0243506

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 33284818

    View Full Text