Sugarcane cystatins: From discovery to biotechnological applications.

International journal of biological macromolecules 2021 Jan 15

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Phytocystatins are tight-binding cysteine protease inhibitors produced by plants. The first phytocystatin described was isolated from Oryza sativa and, since then, cystatins from several plant species were reported, including from sugarcane. Sugarcane cystatins were unraveled in Sugarcane EST project database, after sequencing of cDNA libraries from various sugarcane tissues at different developmental stages and six sugarcane cystatins were cloned, expressed and characterized (CaneCPI-1 to CaneCPI-6). These recombinant proteins were produced in different expression systems and inhibited several cysteine proteases, including human cathepsins B and L, which can be involved in pathologies, such as cancer. In this review, we summarize a comprehensive history of all sugarcane cystatins, presenting an updated phylogenetic analysis; chromosomal localization, and genomic organization. We also present protein docking of CaneCPI-5 in the active site of human cathepsin B, insights about canecystatins structures; recombinant expression in different systems, comparison of their inhibitory activities against human cysteine cathepsins B, K, L, S, V, falcipains from Plasmodium falciparum and a cathepsin L-like from the sugarcane weevil Sphenophorus levis; and enlighten their potential and current applications in agriculture and health. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Priscila Yumi Tanaka Shibao, Célio Dias Santos-Júnior, Adelita Carolina Santiago, Chakravarthi Mohan, Mariana Cardoso Miguel, Danyelle Toyama, Marcos Antonio Sanches Vieira, Subramonian Narayanan, Antonio Figueira, Adriana K Carmona, Andreas Schiermeyer, Andrea Soares-Costa, Flavio Henrique-Silva. Sugarcane cystatins: From discovery to biotechnological applications. International journal of biological macromolecules. 2021 Jan 15;167:676-686