Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

In this review we describe the application of CRISPR tools for functional genomics screens in bacteria, with a focus on the use of interference (CRISPRi) approaches. We review recent developments in CRISPRi titration, which has enabled essential gene functional screens, and genome-scale pooled CRISPRi screens. We summarize progress toward enabling CRISPRi screens in non-model and pathogenic bacteria, including the development of new dCas9 variants. Taking into account the current state of the field, we provide a forward-looking analysis of CRISPRi strategies for determining gene function in bacteria. Copyright © 2020. Published by Elsevier Ltd.

Citation

Horia Todor, Melanie R Silvis, Hendrik Osadnik, Carol A Gross. Bacterial CRISPR screens for gene function. Current opinion in microbiology. 2021 Feb;59:102-109


PMID: 33285498

View Full Text