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In this review we describe the application of CRISPR tools for functional genomics screens in bacteria, with a focus on the use of interference (CRISPRi) approaches. We review recent developments in CRISPRi titration, which has enabled essential gene functional screens, and genome-scale pooled CRISPRi screens. We summarize progress toward enabling CRISPRi screens in non-model and pathogenic bacteria, including the development of new dCas9 variants. Taking into account the current state of the field, we provide a forward-looking analysis of CRISPRi strategies for determining gene function in bacteria. Copyright © 2020. Published by Elsevier Ltd.


Horia Todor, Melanie R Silvis, Hendrik Osadnik, Carol A Gross. Bacterial CRISPR screens for gene function. Current opinion in microbiology. 2021 Feb;59:102-109

PMID: 33285498

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