Separation based characterization methods for the N-glycosylation analysis of prostate-specific antigen.

Balazs Reider, Gabor Jarvas, Jana Krenkova, Andras Guttman

Journal of pharmaceutical and biomedical analysis 2021 Feb 05

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Prostate cancer has the highest malignancy rate diagnosed in men worldwide. Albeit, the gold standard serum prostate-specific antigen (PSA) assays reduced the mortality rate of the disease, the number of false positive diagnoses steeply increased. Therefore, there is an urgent need for complementary biomarkers to enhance the specificity and selectivity of current diagnostic methods. Information about PSA glycosylation can help to fulfill this gap as alterations of its carbohydrate moieties due to cancerous transformation may represent additional markers to distinguish malignant from benign tumors. However, development of suitable methods and instrumentations to investigate the N-glycosylation profile of PSA represents a challenge. In this paper, we critically review the current bioanalytical trends and strategies in the field of PSA glycobiomarker research focusing on separation based characterization methods. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Balazs Reider, Gabor Jarvas, Jana Krenkova, Andras Guttman. Separation based characterization methods for the N-glycosylation analysis of prostate-specific antigen. Journal of pharmaceutical and biomedical analysis. 2021 Feb 05;194:113797