Fabrication of a Novel, Cost-Effective Double-Sided Indium Tin Oxide-Based Nanoribbon Electrode and Its Application of Acute Toxicity Detection in Water.

Dengbin Yu, Youxing Fang, Ling Liu, Jingting He, Xuerong Han, Hongwen Yu, Shaojun Dong

ACS sensors 2020 Dec 24

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Microelectrode plays a crucial role in developing a rapid biosensor for detecting toxicity in water. In this study, a nanoribbon electrode (NRE) with amplified microelectrode signal was successfully prepared by electrodepositing 2-allylphenol on a double-sided indium tin oxide glass. The NRE provided a simple mean for obtaining large steady-state current response. Its advantages were discussed by contrasting the toxicity detection of 3,5-dichlorophenol (DCP) with single microelectrode, microelectrode array, and millimeter electrode as working electrodes in which potassium ferricyanide (K3[Fe(CN)6]) was adopted as a mediator, and Escherichia coli was selected as bioreceptor. At a constant potential of 450 mV, the current reached a steady state within 10 s. The biosensor was constructed using the NRE as working electrode, and its feasibility was verified by determining the toxicity of DCP. A 50% inhibitory concentration (IC50) of 3.01 mg/L was obtained by analyzing the current responses of different concentrations of DCP within 1 h. These results exhibited that the proposed method based on the as-prepared NRE was a rapid, sensitive, and cost-effective way for toxicity detection in water.

Citation

Dengbin Yu, Youxing Fang, Ling Liu, Jingting He, Xuerong Han, Hongwen Yu, Shaojun Dong. Fabrication of a Novel, Cost-Effective Double-Sided Indium Tin Oxide-Based Nanoribbon Electrode and Its Application of Acute Toxicity Detection in Water. ACS sensors. 2020 Dec 24;5(12):3923-3929