Correlation Engine 2.0
Clear Search sequence regions


  • acid (7)
  • agrobacterium (3)
  • Atu1419 (4)
  • crystal (1)
  • dimers (1)
  • family (1)
  • genes (4)
  • GntR (4)
  • HcaR (2)
  • ion (1)
  • models molecular (1)
  • nucleic acids (1)
  • nucleotides (1)
  • plant (1)
  • protein domains (1)
  • represses (1)
  • repressor proteins (2)
  • research (1)
  • sodium (2)
  • tetrahydrofolates (2)
  • zinc (2)
  • Sizes of these terms reflect their relevance to your search.

    A species-specific region, denoted SpG8-1b allowing hydroxycinnamic acids (HCAs) degradation is important for the transition between the two lifestyles (rhizospheric versus pathogenic) of the plant pathogen Agrobacterium fabrum. Indeed, HCAs can be either used as trophic resources and/or as induced-virulence molecules. The SpG8-1b region is regulated by two transcriptional regulators, namely, HcaR (Atu1422) and Atu1419. In contrast to HcaR, Atu1419 remains so far uncharacterized. The high-resolution crystal structures of two fortuitous citrate complexes, two DNA complexes and the apoform revealed that the tetrameric Atu1419 transcriptional regulator belongs to the VanR group of Pfam PF07729 subfamily of the large GntR superfamily. Until now, GntR regulators were described as dimers. Here, we showed that Atu1419 represses three genes of the HCAs catabolic pathway. We characterized both the effector and DNA binding sites and identified key nucleotides in the target palindrome. From promoter activity measurement using defective gene mutants, structural analysis and gel-shift assays, we propose N5,N10-methylenetetrahydrofolate as the effector molecule, which is not a direct product/substrate of the HCA degradation pathway. The Zn2+ ion present in the effector domain has both a structural and regulatory role. Overall, our work shed light on the allosteric mechanism of transcription employed by this GntR repressor. © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

    Citation

    Armelle Vigouroux, Thibault Meyer, Anaïs Naretto, Pierre Legrand, Magali Aumont-Nicaise, Aurélie Di Cicco, Sébastien Renoud, Jeanne Doré, Daniel Lévy, Ludovic Vial, Céline Lavire, Solange Moréra. Characterization of the first tetrameric transcription factor of the GntR superfamily with allosteric regulation from the bacterial pathogen Agrobacterium fabrum. Nucleic acids research. 2021 Jan 11;49(1):529-546

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 33313837

    View Full Text