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Low concentrations of type-I interferon (IFN) in blood seem to be associated with more severe forms of Coronavirus disease 2019 (COVID-19). However, following the type-I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature on a FilmArray® system, which includes PCR assays for four interferon stimulated genes. We analyzed three types of patient populations: (i) children admitted to a pediatric emergency unit for fever and suspected infection, (ii) ICU-admitted patients with severe COVID-19, and (iii) healthcare workers with mild COVID-19. The results were compared to the reference tools, that is, molecular signature assessed with Nanostring® and IFN-α2 quantification by SIMOA® (Single MOlecule Array). A strong correlation was observed between the IR measured by the FilmArray®, Nanostring®, and SIMOA® platforms (r-Spearman 0.996 and 0.838, respectively). The FilmArray® panel could be used in the COVID-19 pandemic to evaluate the IR in 45-min with 2 min hand-on-time at hospitalization and to monitor the IR in future clinical trials. © 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.

Citation

Marine Mommert, Magali Perret, Matthew Hockin, Sébastien Viel, Alexandre Belot, Jean-Christophe Richard, Mehdi Mezidi, Jean-Baptiste Fassier, Etienne Javouhey, ANTOINE Study Group, COVID-SER Study Group, COVID-HCL Study Group, Andrew Hemmert, François Mallet, Sophie Trouillet-Assant, Karen Brengel-Pesce. Type-I Interferon assessment in 45 minutes using the FilmArray® PCR platform in SARS-CoV-2 and other viral infections. European journal of immunology. 2021 Apr;51(4):989-994

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PMID: 33314090

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