Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer.

Variation of DNA conformation is important in regulating gene expression and mediating drug-DNA interactions. However, directly probing transient DNA conformation changes is challenging owing to the dynamic nature of this process. We show a label-free fluorescence method to monitor transient DNA conformation changes in DNA structures with various lengths and shapes using a DNA intercalator, K21. K21 can form transient excimers on the surface of DNA; the ratiometric emission of monomer and excimer correlate to DNA transient conformation stability in numerous DNA structures, including i-motifs, G-quadruplex structures, and single nucleotide mutation at random position. We analyzed the conformation dynamics of a single plasmid before and after enzyme digestion with confocal fluorescence microscopy. This method provides a label-free fluorescence strategy to probe transient conformation changes of DNA structures and has potential in uncovering transient genomic processes in living cells. © 2020 Wiley-VCH GmbH.

Citation

Bin Chen, Qiuling Huang, Zhibei Qu, Cong Li, Qian Li, Jiye Shi, Chunhai Fan, Lihua Wang, Xiaolei Zuo, Jianlei Shen, Jiang Li. Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer. Angewandte Chemie (International ed. in English). 2021 Mar 15;60(12):6624-6630

Mesh Tags

Substances

PMID: 33314629

search → result