Ji-Yeon Kim, Sehoon Park, Jiyeong Jeong, Mungyu Lee, Byeongchan Kang, Se Hwan Jang, Jinsung Jeon, Nulee Jang, Soyoung Oh, Zee-Yong Park, In Seop Chang
Bioresource technology 2021 FebThis study analyzed the effect of methanol on the metabolism of syngas components (i.e., H2 and CO) by the syngas fermenting acetogenic strain E. limosum KIST612. The culture characteristics and relevant proteomic expressions (as fold changes) were carefully analyzed under CO/CO2 and H2/CO2 conditions with and without methanol addition, as well as, under methanol/CO2 conditions. The culture characteristics (specific growth rate and H2 consumption rate) under H2/CO2 conditions were greatly enhanced in the presence of methanol, by 4.0 and 2.7 times, respectively. However, the promoting effect of methanol was not significant under CO/CO2 conditions. Proteomic fold changes in most enzyme expression levels in the Wood-Ljungdahl pathway and chemiosmotic energy conservation also exhibited high correspondence between methanol and H2/CO2 but not between methanol and CO/CO2. These findings suggest the advantages of methanol addition to H2/CO2 for biomass enhancement and faster consumption of gaseous substrates during syngas fermentation. Copyright © 2020 Elsevier Ltd. All rights reserved.
Ji-Yeon Kim, Sehoon Park, Jiyeong Jeong, Mungyu Lee, Byeongchan Kang, Se Hwan Jang, Jinsung Jeon, Nulee Jang, Soyoung Oh, Zee-Yong Park, In Seop Chang. Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612. Bioresource technology. 2021 Feb;321:124521
PMID: 33321298
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