Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma.

Cell proliferation is broadly defined as a process leading to an increase of cell number, essentially depending on a balance between cell cycle progression/cell division, cell death, and cellular senescence. Deregulation of cell proliferation is a key feature of cancer cells, making assessment of proliferation a central methodological issue in cancer research. Especially in Ewing sarcoma (EwS) that exhibit a high proliferative capacity, experimental assessment of proliferation in preclinical research plays an important role. Among the variety of applicable methods, trypan blue exclusion is described here as a robust, easy-to-perform, and cost-effective method to assess cell proliferation in an experimental setting.

Citation

Cornelius Maximilian Funk, Julian Musa. Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma. Methods in molecular biology (Clifton, N.J.). 2021;2226:151-158

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PMID: 33326099

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