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Actinomycin D has long been used as a first-line antitumor drug in clinical practice. Actinomycin X2, a new drug lead, is often isolated along with actinomycin D. The minor differences between the two actinomycin analogs pose a daunting challenge in separation. In this study, supercritical fluid chromatography (SFC) was successfully utilized for the purification of actinomycin X2 and actinomycin D from a marine derived Streptomyces sp. DQS-5. After one-step SFC purification, the purities of these two compounds were determined to be 97.3 % and 97.8 %, respectively. This method provides a green alternative for the separation of these pharmacologically important actinomycin antibiotics. This study also demonstrated the development of a simple and rapid method for the separation of natural products based on SFC. Copyright © 2020 Elsevier B.V. All rights reserved.


Xiao-Jie Lu, Na Du, Da-Bing Zhang, Xiao-Xue Zhou, Xiao-Fan Li, Jian-Hua Ju, Zhang-Li Hu, Li-Yan Wang. Efficient, green, and rapid strategy for separating actinomycin D and X2 using supercritical fluid chromatography. Journal of pharmaceutical and biomedical analysis. 2021 Feb 20;195:113835

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PMID: 33328146

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