High-purity DNA extraction from animal tissue using picking in the TRIzol-based method.

Ye Yin Bo, Li Dong Liang, Yang Jiang Hua, Zhang Zhao, Mei Si Yao, Liu Bao Shan, Chen Ze Liang

BioTechniques 2021 Mar

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TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the picking method's DNA is ideal and better than that obtained by the classic TRIzol method. Gel electrophoresis showed that there was no RNA contamination, and the DNA had not degraded. DNA extracted by the picking method had the same performance in restriction enzyme digestion and quantitative PCR as that obtained by the traditional method. Viral DNA in the infected tissue was also obtained. This modified method facilitates various molecular biology assays.

Citation

Ye Yin Bo, Li Dong Liang, Yang Jiang Hua, Zhang Zhao, Mei Si Yao, Liu Bao Shan, Chen Ze Liang. High-purity DNA extraction from animal tissue using picking in the TRIzol-based method. BioTechniques. 2021 Mar;70(3):186-190