In vitro susceptibility of Mycobacterium abscessus complex and feasibility of standardizing treatment regimens.

Ka Lip Chew, Sophie Octavia, Joelle Go, Sally Ng, Yit Er Tang, Patsy Soh, Joy Yong, Roland Jureen, Raymond Tzer Pin Lin, Siang Fei Yeoh, Jeanette Teo

The Journal of antimicrobial chemotherapy 2021 Mar 12

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To determine the in vitro susceptibility of members of the Mycobacterium abscessus complex to routinely tested antibiotics and to an extended antibiotic panel. Non-duplicate isolates for which susceptibility testing results were available were included in this study. Retrospective laboratory records were reviewed, including tigecycline susceptibility results, and testing was performed with additional drugs, including vancomycin, dalbavancin, telavancin, oritavancin, rifabutin, delafloxacin, eravacycline, clofazimine and bedaquiline using broth microdilution (Sensititre, Thermo Fisher). A total of 218 M. abscessus complex isolates were included for retrospective review, of which 151 were respiratory isolates. Of these 218 isolates, 211 were available for additional testing with the extended antibiotic panel. Of these, 146 were respiratory isolates. One isolate had a vancomycin MIC of 2 mg/L and MICs of all other isolates were >8 mg/L. All isolates had MICs of >8 mg/L for oritavancin, dalbavancin and telavancin. One isolate had a delafloxacin MIC of 4 mg/L and MICs of all other isolates were >8 mg/L. The MIC50/MIC90s of rifabutin, tigecycline, eravacycline, clofazimine and bedaquiline were 16/32, 0.5/1, 0.12/0.25, 0.12/0.25 and 0.06/0.12 mg/L, respectively. In vitro activity was demonstrated for clofazimine, bedaquiline and eravacycline, indicating potential for inclusion as standardized therapy for M. abscessus complex infections. © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Citation

Ka Lip Chew, Sophie Octavia, Joelle Go, Sally Ng, Yit Er Tang, Patsy Soh, Joy Yong, Roland Jureen, Raymond Tzer Pin Lin, Siang Fei Yeoh, Jeanette Teo. In vitro susceptibility of Mycobacterium abscessus complex and feasibility of standardizing treatment regimens. The Journal of antimicrobial chemotherapy. 2021 Mar 12;76(4):973-978