A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization.

Amy E Emerson, Emily M Slaby, Jessica D Weaver

Methods in molecular biology (Clifton, N.J.) 2021

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As the field of organoid development matures, the need to transplant organoids to evaluate and characterize their functionality grows. Decades of research developing islet organoid transplantation for the treatment of type 1 diabetes can contribute substantially to accelerating diverse tissue organoid transplantation. Biomaterials-based organoid delivery methods offer the potential to maximize organoid survival and engraftment. In this protocol, we describe a vasculogenic degradable hydrogel vehicle and a method to deliver organoids to intraperitoneal tissue. Further, we describe a method to fluorescently label and image functional vasculature within the graft as a tool to investigate organoid engraftment.

Citation

Amy E Emerson, Emily M Slaby, Jessica D Weaver. A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization. Methods in molecular biology (Clifton, N.J.). 2021;2258:259-272