The B″-family subunits of protein phosphatase 2A are necessary for in-vitro dephosphorylation of the Arabidopsis mechanosensory transcription factor VIP1.

Hyuk Sung Yoon, Kaien Fujino, Shenkui Liu, Tetsuo Takano, Daisuke Tsugama

Biochemical and biophysical research communications 2021 Jan 01

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Protein phosphatase 2A (PP2A) B″-family subunits have Ca2+-binding EF-hand motifs and can bind PP2A substrates. Arabidopsis thaliana PP2A B″-family subunits are encoded by six genes, and bind a transcription factor, VIP1. VIP1 is dephosphorylated and nuclear-localized by hypo-osmotic stress. However, whether PP2A B″-family subunits mediate the VIP1 dephosphorylation is unclear. Here, we show by yeast two-hybrid and in vitro pull down assays that Arabidopsis PP2A B″-family subunits bind Arabidopsis PP2A A (scaffold) subunits. We also show that VIP1 dephosphorylation in vitro can be induced by a PP2A B″-family subunit. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Hyuk Sung Yoon, Kaien Fujino, Shenkui Liu, Tetsuo Takano, Daisuke Tsugama. The B″-family subunits of protein phosphatase 2A are necessary for in-vitro dephosphorylation of the Arabidopsis mechanosensory transcription factor VIP1. Biochemical and biophysical research communications. 2021 Jan 01;534:353-358