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To study the effect of mahogunin ring finger-1 (MGRN1) on the autophagy of Sertoli cells in mice. Using RNA interference, we down-regulated the expression of MGRN1 in the mouse TM4 Sertoli cells cultured in vitro, determined the expressions of the autophagy-related proteins LC3-II/I, ATG-5 and ATG-7 by Western blot, and detected the autophagosomes in the TM4 cells by immunofluorescence and electron microscopy. Western blot showed increased expressions of LC3-II/I, ATG-5 and ATG-7 in the mouse TM4 Sertoli cells after knockdown of MGRN1. Fluorescence microscopy revealed significantly more autophagosomes in the TM4 cells than in the control group (P < 0.05). MGRN1 affects the autophagy of mouse Sertoli cells, and its specific molecular mechanism needs to be further studied.

Citation

Yuan Zhu, Yong Luo, Hai-Yuan Lu, Fa-Ying Liu, Li-Qun Wang. Influence of MGRN1 on the autophagy of Sertoli cells in mice]. Zhonghua nan ke xue = National journal of andrology. 2020 Apr;26(4):297-302

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PMID: 33351294

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