Associations between red blood cells fatty acids, desaturases indices and metabolism of platelet activating factor in healthy volunteers.

E Fragopoulou, P Detopoulou, E Alepoudea, T Nomikos, N Kalogeropoulos, S Antonopoulou

Prostaglandins, leukotrienes, and essential fatty acids 2021 Jan

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Platelet-activating-factor is an inflammatory lipid mediator. Key enzymes of its biosynthesis are CDP-choline:1-alkyl-2-acetyl-sn-glycerol-cholinephosphotransferase (PAF-CPT) and acetyl-CoA:lyso-PAF-acetyltransferases (Lyso-PAF-AT) while PAF-AH/Lp-PLA2 degrade PAF. The interplay between PAF and fatty acids metabolism was explored. In a healthy population, PAF levels, its metabolic enzymes activity and RBC fatty acids were measured while desaturases indices (D) were estimated. A principal component analysis was also applied to assess patterns of RBC fatty acids. SFA were related to increased PAF biosynthesis and decreased Lp-PLA2 only in women. MUFA were inversely associated with PAF biosynthesis and positively with Lp-PLA2. Omega-6 fatty acids were positively correlated only with PAF-CPT while no significant correlations were observed with n3 fatty acids. D6 index was positively related with PAF biosynthetic enzymes and inversely with Lp-PLA2 while D9 correlated positively with Lp-PLA2. The pattern of high MUFA and low n6 was associated with reduced PAF biosynthesis and/or increased catabolism in both sexes. The role of fatty acids in amplifying or reducing inflammation seems to be also reflected in PAF metabolism. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

E Fragopoulou, P Detopoulou, E Alepoudea, T Nomikos, N Kalogeropoulos, S Antonopoulou. Associations between red blood cells fatty acids, desaturases indices and metabolism of platelet activating factor in healthy volunteers. Prostaglandins, leukotrienes, and essential fatty acids. 2021 Jan;164:102234