Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts.

Sebastian Reusch, Abin Biswas, William Graham Hirst, Simone Reber

STAR protocols 2020 Dec 18

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Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020). © 2020 The Authors.

Citation

Sebastian Reusch, Abin Biswas, William Graham Hirst, Simone Reber. Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts. STAR protocols. 2020 Dec 18;1(3):100151