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Based on the green chemistry approach, highly fluorescent and novel carbon dots (C. dots) were synthesized from naturally available and cost effective sunflower seeds. The obtained C. dots showed a fluorescence quantum yield (Q.Y) of 9.5% with high water dispersibility and photostability. The obtained C. dots were employed for the detection of picric acid (PA) and bilirubin. A good linear relationship in the range of 20-60 nM was obtained for PA with a limit of detection (LOD) as low as 3.86 nM. C. dots were successfully incorporated in the agarose matrix which enabled them to be employed as a solid platform for the in situ detection of PA. The fluorescence of C. dots was selectively quenched by bilirubin compared to other biomolecules with a LOD of 2.03 μM. Use of C. dots as potential candidate for bilirubin detection was verified by real sample analysis. Further, the separation of C. dots was performed using column chromatography and the optical properties of the two different fractions obtained were studied. The blue fraction of C. dots was found to exhibit a higher fluorescence Q.Y and excitation independent emission, with an improved detection of PA and bilirubin. Copyright © 2020 Elsevier B.V. All rights reserved.


V Roshni, Varsha Gujar, Syed Muntjeeb, Pooja Doshi, Divya Ottoor. Novel and Reliable Chemosensor Based on C. dots from Sunflower seeds for the Distinct Detection of Picric Acid and Bilirubin. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2021 Apr 05;250:119354

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PMID: 33387803

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