Evaluation of Conversion Factors for Genetically Modified Maize Quantification].

Reona Takabatake, Mari Onishi, Junichi Mano, Masahiro Kishine, Keisuke Soga, Kosuke Nakamura, Kazunari Kondo, Kazumi Kitta

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan 2020

filter terms:

To quantify the amount of authorized GM maize or soybean, conversion factor (Cf) values are required for converting the copy number ratio of GM sequence to an endogenous sequence into weight-based GMO amounts. Cf values are available for the several latest real-time PCR instruments such as QuantStudio5, QuantStudio12K Flex, LightCycler 96, and LightCycler 480 for GM soybeans but not for GM maize. For the quantification of GM maize, we experimentally determined the Cf values targeting Cauliflower mosaic virus 35S promoter (P35S), GA21 construct specific, MIR604 event specific and MIR162 event specific sequences using the four real-time PCR instruments.

Citation

Reona Takabatake, Mari Onishi, Junichi Mano, Masahiro Kishine, Keisuke Soga, Kosuke Nakamura, Kazunari Kondo, Kazumi Kitta. Evaluation of Conversion Factors for Genetically Modified Maize Quantification]. Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan. 2020;61(6):235-238