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Molecular hydrogen (H2 ) has been reported to have important biological effects on bone tissue in several in vitro and in vivo models. Danio rerio (zebrafish) embryo is a good model to study osteogenesis because of its transparency, size and rapid development. In zebrafish embryo, hydrogen-rich water (HRW) does not affect vitality or growth rate up to 15%. In addition, 7% HRW treatment enhances zebrafish embryo osteogenesis, increasing the vertebral mineralization rate. In conclusion, we demonstrated the beneficial effects of hydrogen on anabolic bone functions in vivo. © 2021 Fisheries Society of the British Isles.


Marta Carnovali, Massimo Mariotti, Giuseppe Banfi. Molecular hydrogen enhances osteogenesis in Danio rerio embryos. Journal of fish biology. 2021 May;98(5):1471-1474

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PMID: 33410131

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