Serological and molecular study of a novel B(A) allele with multiple missense mutations].

To explore the molecular basis for an individual suspected as AwB subtype through DNA sequencing. ABO serology was carried out with the standard tube method. To identify the ABO gene haplotype, the amplicons of exon 7 were cloned and sequenced. Serological results showed that the forward typing was AwB and the reverse typing was B. Sequencing analysis revealed that the sample has contained an O01 allele in addition with c.297A>G, c.657C>T, c.796C>A, c.803G>C, c.930G>A variants as compared with the A101 allele. Through sequencing analysis, the sample with an AwB subtype by serological testing was identified as a novel B(A) phenotype, which was unreported previously.

Citation

Bin Han, Shaoqiang Zhang, Yuli Zhu, Zhihui Feng, Shutao Pang. Serological and molecular study of a novel B(A) allele with multiple missense mutations]. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics. 2021 Jan 10;38(1):20-22

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PMID: 33423251

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