Enrichment of antibiotic resistance genes after sheep manure aerobic heap composting.

Guoying Wang, Guoxue Li, Jiali Chang, Yilin Kong, Tao Jiang, Jiani Wang, Jing Yuan

Bioresource technology 2021 Mar

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In this study, physio-chemical properties, 45 antibiotics, 6 heavy metals, 42 antibiotic resistance genes (ARGs), 3 mobile genetic elements, and the bacterial community structure were investigated to analyze the fate of ARGs during sheep manure aerobic heap composting. Results showed that sheep manure heap composting could produce mature compost. The degradation processes reduced the total antibiotics content by 85%. The abundance of ARGs and mobile genetic elements (MGEs) were enriched 9-fold, with the major increases to sul and tet genes (sulI, sulII, tetQ, and tetX). Tetracycline and sulfonamide resistance genes were the most abundant ARGs after composting (more than 88% of all genes). The genes tetA, tetX and sulI were related to the most diverse bacteria that were most able to proliferate during heap composting. Therefore, sulI and tetX are the major ARGs to be controlled, and Actinobacteria and Bacteroidetes may be the major host bacteria. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Guoying Wang, Guoxue Li, Jiali Chang, Yilin Kong, Tao Jiang, Jiani Wang, Jing Yuan. Enrichment of antibiotic resistance genes after sheep manure aerobic heap composting. Bioresource technology. 2021 Mar;323:124620