Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training.

A S Deshmukh, D E Steenberg, M Hostrup, J B Birk, J K Larsen, A Santos, R Kjøbsted, J R Hingst, C C Schéele, M Murgia, B Kiens, E A Richter, M Mann, J F P Wojtaszewski

Nature communications 2021 Jan 12

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Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca2+ signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.

Citation

A S Deshmukh, D E Steenberg, M Hostrup, J B Birk, J K Larsen, A Santos, R Kjøbsted, J R Hingst, C C Schéele, M Murgia, B Kiens, E A Richter, M Mann, J F P Wojtaszewski. Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training. Nature communications. 2021 Jan 12;12(1):304